Journal: Plant Methods

Article Title: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture

doi: 10.1186/s13007-023-01018-w

Figure Lengend Snippet: Experimental setup of the phenotyping system designed for direct monitoring of plantlets and explants cultured in vitro. A 3D representation of the designed robot platform inside a multi-layered shelf system with bottom water cooling. B Closeup of sensor arrangement of the developed multi-sensor detector head. Four different sensors, including C a laser distance sensor, D RGB camera, E a micro spectrometer and F a thermal camera defined the multi-sensor detector head. Furthermore, G a ring-light printed circuit board, including UV, white and red LEDs was added to a purchasable diffuse ring light to meet the highly specific illumination situation of monitoring plant in vitro cultures. Detailed description in “ ” section

Article Snippet: Colored rectangles indicate waveband regions and spectral sensitivity of the sensors (according to the manufacturer’s specifications) installed in the phenotyping platform (blue, RGB camera with Sony IMX 477 sensitivity: 400 to 700 nm; green, micro spectrometer sensitivity: 340 to 850 nm; red, L, Laser distance sensor emission wavelength: 655 nm; brown, thermal camera sensitivity: 8000 to 14,000 nm) While all of the three tested sealings had high transmittance (> 91%) in the visible spectrum (VIS) (Fig. A), the tested materials differed strongly in the proportion of transmitted diffuse light (Table ).

Techniques: Cell Culture, In Vitro

Journal: Plant Methods

Article Title: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture

doi: 10.1186/s13007-023-01018-w

Figure Lengend Snippet: Spectral transmittance of culture vessel closures. Transmittance was measured with A an UV/VIS/NIR spectrometer and B with a FT-IR Spectrometer. Three independent replicates were measured and mean spectra per lid material are shown. Colored rectangles indicate waveband regions and spectral sensitivity of the sensors (according to the manufacturer’s specifications) installed in the phenotyping platform (blue, RGB camera with Sony IMX 477 sensitivity: 400 to 700 nm; green, micro spectrometer sensitivity: 340 to 850 nm; red, L, Laser distance sensor emission wavelength: 655 nm; brown, thermal camera sensitivity: 8000 to 14,000 nm)

Article Snippet: Colored rectangles indicate waveband regions and spectral sensitivity of the sensors (according to the manufacturer’s specifications) installed in the phenotyping platform (blue, RGB camera with Sony IMX 477 sensitivity: 400 to 700 nm; green, micro spectrometer sensitivity: 340 to 850 nm; red, L, Laser distance sensor emission wavelength: 655 nm; brown, thermal camera sensitivity: 8000 to 14,000 nm) While all of the three tested sealings had high transmittance (> 91%) in the visible spectrum (VIS) (Fig. A), the tested materials differed strongly in the proportion of transmitted diffuse light (Table ).

Techniques:

Journal: Plant Methods

Article Title: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture

doi: 10.1186/s13007-023-01018-w

Figure Lengend Snippet: Potential applications of the automated low-cost phenotyping system in plant vitro culture. While the requirements for the use cases from A – C , E and H have already been met, further research is required for the use cases D , F to G (External images from Yuan et al. and Quambusch et al. )

Article Snippet: Colored rectangles indicate waveband regions and spectral sensitivity of the sensors (according to the manufacturer’s specifications) installed in the phenotyping platform (blue, RGB camera with Sony IMX 477 sensitivity: 400 to 700 nm; green, micro spectrometer sensitivity: 340 to 850 nm; red, L, Laser distance sensor emission wavelength: 655 nm; brown, thermal camera sensitivity: 8000 to 14,000 nm) While all of the three tested sealings had high transmittance (> 91%) in the visible spectrum (VIS) (Fig. A), the tested materials differed strongly in the proportion of transmitted diffuse light (Table ).

Techniques:

Journal: Plant Methods

Article Title: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture

doi: 10.1186/s13007-023-01018-w

Figure Lengend Snippet: Software design and network communication of the phenotyping system components. Two Raspberry Pis hosting Docker containers executing scripts for the two main tasks of motion control and sensor control and providing the access over HTTP request via the Python framework FastAPI by the main script (semi-autonomous mode/local communication) or the user (reliant mode/ wireless communication). Gray areas represent physically co-located software elements, arrows indicate the direction of data transfer, and black lines mark physically connected hardware components

Article Snippet: Colored rectangles indicate waveband regions and spectral sensitivity of the sensors (according to the manufacturer’s specifications) installed in the phenotyping platform (blue, RGB camera with Sony IMX 477 sensitivity: 400 to 700 nm; green, micro spectrometer sensitivity: 340 to 850 nm; red, L, Laser distance sensor emission wavelength: 655 nm; brown, thermal camera sensitivity: 8000 to 14,000 nm) While all of the three tested sealings had high transmittance (> 91%) in the visible spectrum (VIS) (Fig. A), the tested materials differed strongly in the proportion of transmitted diffuse light (Table ).

Techniques: Software, Control